Affiliations: Institute of Photonic Technology, Jena, Germany | University of St. Andrews, School of Physics and Astronomy, United Kingdom | Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University of Jena, Jena, Germany
Note: [] Corresponding author: Christoph Krafft, Institute of Photonic Technology, Albert Einstein Straße 9, 07745 Jena, Germany. E-mail: christoph.krafft@ipht-jena.de
Abstract: We demonstrate that wavelength modulation overcomes frequent obstacles for in-situ and in-vivo implementations of Raman spectroscopy, namely autofluorescence, removal of the system transmission function including etaloning as well as compensation of ambient light. It is the method of choice for Raman measurements of samples with high background under ambient light conditions such as real-time assessment of tissues in the operating theatre.
