The influence of the cytokines Il‐1\beta and INF\gamma on the expression of selenoproteins in the human hepatocarcinoma cell line HepG2

Article type: Abstract

Authors: Hesse‐Bähr, K. | Dreher, I. | Köhrle, J.^;

Affiliations: Klinische Forschergruppe der Medizinischen Poliklinik der Universität Würzburg, Röntgenring 11, D‐97070 Würzburg, Germany

Note: [] Address correspondence to: Prof. Dr Josef Köhrle, Abteilung für Molekulare Innere Medizin und Klinische Forschergruppe der Medizinischen Poliklinik der Universität Würzburg, Röntgenring 11, D‐97070 Würzburg, Germany. Tel.: +49 931 201 7101; Fax: +49 931 2017107.

Abstract: Metabolic labeling of HepG2 cells with ^{75}selenite indicated the expression of more than four selenoproteins in the 80 to 45 kDa range and more than four selenoproteins in the 24 to 14 kDa range. Although there were no significant changes in the total protein content, determined by the method of BioRad, the densitometric analysis of the autoradiogramms showed a reduced expression of all labeled selenoproteins in the cell lysates of HepG2 after cytokine treatment for 24 hours. A stronger reduction was observed after treatment with Il‐1\beta than with IFN\gamma. Moreover, the inhibitory effects were more significant for those selenoproteins in the higher molecular mass range. Our data on the inhibition of selenoprotein synthesis and on repression of SeP promoter activity show that the expression of selenoproteins, especially of SeP, is influenced by acute phase reaction and pro‐inflammatory reactions.

Journal: Biofactors, vol. 11, no. 1-2, pp. 83-85, 2000