Affiliations: Department of Animal Biology II (Animal Physiology), Faculty of Biology, Complutense University, Madrid, Spain
Note: [] Address correspondence to: M. De la Fuente, Departamento de Biología Animal II (Fisiología Animal), Facultad de Biología, Universidad Complutense de Madrid, Avda. Complutense, s/n 28040 Madrid, Spain. Fax: +34 1 394 4935; E‐mail: rafacr@arrakis.es.
Abstract: A previous study has shown that diet supplementation with thioproline (thiazolidine‐4‐carboxylic acid, TCA), an intracellular sulfhydril antioxidant and free radical scavenger, stimulates lymphocyte functions in old mice. In the present work, the effect of thioproline ingestion on the phagocytic functions of peritoneal macrophages, namely adherence, chemotaxis, phagocytosis and superoxide anion production was studied in adult female OF1‐Swiss mice, that were fed thioproline (0.1% w/w) for five weeks, starting this ingestion at the age of 22 \pm 2 weeks. Mice were divided into a fast and a slow group based on their exploratory activity, which was assessed by their performance in a T‐shaped maze. Slow mice showed a worse phagocytic activity with respect to fast animals. After thioproline treatment, a stimulation of all the functions studied as well as a neutralization of the superoxide radical were observed. The effect of this antioxidant was stronger in the slow than in the fast group. Thus, a diet supplemented with thioproline may enhance the immune functions, especially when they are depressed.
