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Article type: Research Article
Authors: Schulte, Ulrich; | Abelmann, Anke | Amling, Natascha | Brors, Benedikt | Friedrich, Thorsten | Kintscher, Lars | Rasmussen, Tim | Weiss, Hanns
Affiliations: Institut für Biochemie, Heinrich‐Heine‐Universität Düsseldorf, Universitätsstraße 1, D‐40225 Düsseldorf, Germany
Note: [] Corresponding author: Dr. Ulrich Schulte, Heinrich‐Heine‐Universität Düsseldorf, Institut für Biochemie, Universitätsstrasse 1, D‐40225 Düsseldorf, Germany. Tel.: +49 211 81 12020; Fax: +49 211 81 15310; E‐mail: ulrich.schulte@uni‐duesseldorf.de.
Abstract: The proton‐translocating NADH:ubiquinone oxidoreductase of mitochondria (complex I) is a large L‐shaped multi‐subunit complex. The peripheral matrix arm contains one FMN and a number of iron‐sulfur (FeS) clusters and is involved in NADH oxidation and electron transfer to the membrane intrinsic arm. There, following a yet unknown mechanism, the redox‐driven proton translocation and the ubiquinone reduction take place. Redox groups that would be able to link electron transfer with proton translocation have not been found so far in the membrane arm. We searched for such groups in complex I isolated from Neurospora crassa. Under anaerobic conditions, the preparation was analyzed in different redox states by means of UV/VIS and EPR spectroscopy. Absorption bands in the UV/VIS redox difference spectra were found which cannot be attributed to the FMN or the EPR detectable FeS clusters. The existence of two novel groups is postulated and their possible locations in the electron pathway and their roles in proton translocation are discussed.
Keywords: NADH:ubiquinone oxidoreductase, quinoprotein, iron‐sulfur cluster, mitochondria, respiratory chain, EPR spectroscopy, proton translocation
Journal: Biofactors, vol. 8, no. 3-4, pp. 177-186, 1998
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