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Article type: Research Article
Authors: Mohamed, S.F. | Gvozdiak, O.R. | Stallmann, D. | Griepenburg, U. | Follmann, H. | Auling, G.;
Affiliations: Institut für Mikrobiologie der Universität, D‐30167 Hannover, Germany | Institute of Microbiology and Virology, National Academy of Science of Ukraine, Kiev, Ukraine | Fachbereich Biologie–Chemie der Universität, D‐34109 Kassel, Germany
Note: [] Corresponding author. Fax: +49 511 7625287.
Abstract: The reduction of 2'‐ribonucleotides to 2'‐deoxyribonucleotides, a unique step in DNA formation, is catalyzed by ribonucleotide reductase (RRase), an allosterically regulated, cell cycle‐dependent enzyme. This work reports a reversible impairment of DNA formation and ribonucleotide reduction upon manganese depletion in Bacillus subtilis demonstrated through in vivo labeling with nucleic acid precursors and enzyme assays with ether‐permeabilized cells. No deoxyadenosylcobalamin‐dependent reduction of ribonucleotides was detected in the cytosol, and the properties of a partially purified enzyme fraction, i.e., sensitivity towards EDTA and hydroxyurea (HU), indicated a metal‐dependent type of RRase. The enzyme was enriched by gel filtration on Superose^{\rm TM} 12 from glycerol‐ or fumarate‐grown cells and submitted to Q‐band electron paramagnetic resonance (EPR) spectroscopy for further characterization of the metal center. A distinct Mn(II) signal was obtained in both preparations characteristic of a protein‐bound manganese in a mononuclear metal center with axial symmetry. The intensity of this Mn signal was not affected by addition of the radical scavenger HU (10 mM) but reduced in the presence of 2.5 mM EDTA. On the basis of these results, we suggest that Bacillus subtilis has a Mn‐dependent ribonucleotide reductase.
Journal: Biofactors, vol. 7, no. 4, pp. 337-344, 1998
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