The effect of biothiol on UV irradiated α-lipoic acid

Issue title: Integrated Functions of Diet in Anti-Aging and Cancer Prevention: Papers from the 4th International Niigata Symposium on Diet and Health, 20–30 November 2008, Niigata, Japan

Article type: Research Article

Authors: Wada, Naoki | Wakami, Hirotaka | Konishi, Tetsuya | Matsugo, Seiichi

Affiliations: School of Natural System, College of Science and Engineering, Kanazawa University, Kakuma, Japan | Faculty of Applied Life Sciences, Niigata University of Pharmacy & Applied Life Sciences, Niigata, Japan

Note: [] Address for correspondence: Seiichi Matsugo, School of Natural System, College of Science and Engineering, Kanazawa University, Kakuma, 920-1192, Japan. Tel.: +81 76 264 6219, Fax: +81 76 234 4829; E-mail: matsugoh@t.kanazawa-u.ac.jp

Abstract: α-Lipoic acid (LA) and its reduced form dihydrolipoic acid (DHLA) are well known as strong antioxidants. LA has the characteristic distorted five membered 1,2-dithiolane ring, which is quite vulnerable to UV irradiation. The UV induced decomposition of LA and the effect of the following addition of biothiols to the photoirradiated solution of LA were investigated by UV-vis spectrometry and ^{1}H NMR spectral analysis. The mechanism of UV irradiated LA decomposition was proposed based on the results obtained from NMR and GPC measurements.

Keywords: α-lipoic acid, biothiol, disulfide interchange reaction

DOI: 10.3233/BIO-2009-1082

Journal: BioFactors, vol. 34, no. 4, pp. 285-292, 2008