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Article type: Research Article
Authors: Lehmann, Lutz E. | Weber, Stefan U. | Fuchs, Dagmar | Book, Malte | Klaschik, Sven | Schewe, Jens-Christian | Hoeft, Andreas | Stüber, Frank
Affiliations: Klinik und Poliklinik für Anästhesie und operative Intensivmedizin, Universität Bonn, Bonn, Germany | Department of Anaesthesiology and Pain Therapy, University Hospital Bern "Inselspital", Bern, Switzerland
Note: [] Address for correspondence: Lutz Eric Lehmann, M.D., Klinik und Poliklinik für Anästhesie und operative Intensivmedizin, Universität Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany. Tel.: +49 228 2871 6557; Fax: +49 228 2871 6754; E-mail: Lutz.Lehmann@ukb.uni-bonn.de
Abstract: The oxidoreductase Macrophage Migration Inhibitory Factor (MIF) is discussed as a promising target for immunomodulatory therapy in patients with severe sepsis. Moreover, MIF expresses tautomerase as well as thiol-protein oxidoreductase activities and has a potential role in cellular redox homeostasis, apoptosis inhibition, endotoxin responsiveness as well as regulation of nuclear transcription factors. To further elucidate a potential role of intracellular MIF in severe sepsis, we assessed alterations of intracellular MIF content in peripheral blood leukocytes of patients with severe sepsis in comparison to healthy controls and non-septic patients after major surgery. Intracellular MIF was significantly elevated simultaneously in lymphocytes, B-cells, macrophages and granulocytes of patients with severe sepsis when compared to healthy control individuals (p < 0.05) and increased when compared to non-septic patients after major surgery. In parallel, plasma MIF levels were elevated in severe sepsis (p < 0.05). There was no difference of intracellular MIF in lymphocytes, B-cells, macrophages or granulocytes between surviving and non-surviving patients with severe sepsis (p > 0.05). However, in survivors LPS ex vivo stimulation increased MIF secretion but not in non-survivors of sepsis (p < 0.05). This finding underlines the role of intracellular MIF in inflammatory diseases. It suggests monitoring of intracellular MIF in further clinical and non-clinical research valuable.
Keywords: MIF, FACS, intracellular detection, severe sepsis, cytokine
Journal: BioFactors, vol. 33, no. 4, pp. 281-291, 2008
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