Affiliations: Institute of Human Nutrition and Food Science,
Molecular Nutrition, Christian-Albrechts-University of Kiel, Germany | Institute of Human Nutrition and Food Science, Food
Science, Christian-Albrechts-University of Kiel, Germany
Note: [] Address for correspondence: Frank Döring, Institute of Human
Nutrition and Food Science, Molecular Nutrition, Christian-Albrechts-University
of Kiel, Heinrich-Hecht-Platz 10, 24118 Kiel, Germany. Tel.: +49 431 880 3387;
Fax: +49 431 880 5658; E-mail: sek@molnut.uni-kiel.de
Abstract: Coenzyme Q_{10} (CoQ_{10}) is
an obligatory element in the mitochondrial electron transport system and
functions as a potent antioxidant of lipid membranes. In-vivo and
in-vitro studies indicate an involvement of CoQ_{10} in
inflammatory pathways. Here we studied in the human monocytic cell-line THP-1
the influence of CoQ_{10} on LPS-induced secretion of the
pro-inflammatory chemokines Macrophage inflammatory protein-1 alpha
(MIP-1α), Regulated upon activation, normal T cell
expressed and secreted (RANTES) and Monocyte chemoattractant protein-1 (MCP-1).
In comparison to unstimulated cells, LPS leads to 22-, 3- and 4.5-fold higher
levels of MIP-1α, RANTES and MCP-1 in the cell culture
medium, respectively. Pre-incubation of cells with 10 μM
CoQ_{10} resulted in a significant decrease of LPS-induced
MIP-1α and RANTES secretion to 55.04% (p = 0.02)
and 76.84% (p = 0.04), respectively. In conclusion,
CoQ_{10} reduces the LPS-induced secretion levels of the
pro-inflammatory chemokines MIP-1α and RANTES in the
human monocytic cell line THP-1. These data suggest that
CoQ_{10} possesses anti-inflammatory properties.
