Amadori-glycated albumin-induced vascular smooth muscle cell
proliferation and expression of inhibitor of apoptosis protein-1 and nerve
growth factor-γ
Affiliations: Department of Internal Medicine, Hallym University,
Chuncheon, Korea | Division of Natural Sciences, Kyonggi University,
Suwon, Korea
Note: [] Address for correspondence: Sung-Hee Ihm, M.D., Department of
Internal Medicine, Hallym University Sacred Heart Hospital, 896 Pyungchon-dong,
Dongan-gu, Anyang, Kyonggi-do, 431-070, South Korea. Tel.: +82 31 380 3714;
Fax: +82 31 386 2269; E-mail: ihmsh@hallym.ac.kr
Abstract: We investigated the effects of Amadori-glycated serum albumin (GSA)
on cell proliferation as well as expressions of antioxidant enzyme genes and
marker genes associated with signal transduction pathways in rat aortic
vascular smooth muscle cells (VSMCs). Quiescent VSMCs treated with GSA
(0–500 μg/mL, 48 h) exhibited a dose-dependent increase
in proliferation that was prevented by PD98059 (25 μM),
suggesting a MAPK-dependent signaling pathway. Compared with bovine serum
albumin (BSA)-treated cells, the GSA (500 μg/mL,
24~h)-treated VSMCs showed a higher superoxide dismutase 2 gene expression in
quantitative RT-PCR, suggesting the involvement of oxidative stress. In a
focused oligonucleotide array containing 96 signal transduction-related genes,
expression of inhibitor of apoptosis protein-1 (IAP-1), nerve growth factor-γ
(NGF-γ), and c-jun genes
was significantly higher in the GSA-treated VSMCs. These results suggest that
induction of antiapoptotic proteins like IAP-1 and strong mitogens like
NGF-γ by GSA might further contribute to the VSMC
proliferation and accelerated vascular remodeling in diabetes.
