Affiliations: Institute of Human Nutrition and Food Science,
Molecular Nutrition, Christian-Albrechts-University of Kiel, Germany | Institute of Human Nutrition and Food Science, Food
Science, Christian-Albrechts-University of Kiel, Germany | Vestische Kinder- und Jugendklinik Datteln, University
of Witten/Herdecke, Germany
Note: [] Address for correspondence: Frank Döring, Institute of
Human Nutrition and Food Science, Molecular Nutrition,
Christian-Albrechts-University of Kiel, Heinrich-Hecht-Platz 10, 24118 Kiel,
Germany. Tel.: +49 431 880 3387; Fax: +49 431 880 5658; E-mail:
schmelzer@molnut.uni-kiel.de
Abstract: Studies in humans and cell culture as well as bioinformatics
suggested that Coenzyme Q_{10} (CoQ_{10})
functions as an anti-inflammatory molecule. Here we studied the influence of
CoQ_{10} (Kaneka
Q_{10}™ on secretion of the
pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-α) by using
the human and murine monocytic cell lines THP-1 and RAW264.7 expressing human
apolipoprotein E3 (apoE3) or pro-inflammatory apoE4. Incubation of cells with
physiological (0.1–10 μM) and supra-physiological (> 10 to < 100
μM) concentrations of CoQ_{10} led to an intracellular
accumulation of its reduced form without any cytotoxic effects. Stimulation of
cell models with lipopolysaccharide (LPS) resulted in a substantially release
of TNF-α. When THP-1 cells were pre-incubated with 10 μM
CoQ_{10}, the LPS-induced TNF-α release was
significantly decreased to 72 ± 32%. This effect is similar to those
obtained by 10 μM N-Acetyl-Cysteine, a well known reference antioxidant. In
RAW264.7-apoE3 and -apoE4 cells, significant reductions of LPS-induced
TNF-α secretion to 73.3 ± 2.8% and 74.7 ± 8.9% were found
with 2.5 μM and 75 μM CoQ_{10}, respectively. In
conclusion, CoQ_{10} has moderate anti-inflammatory effects
in two monocytic cell lines which could be mediated by its antioxidant
activity.
