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Issue title: Plant and Animal Cell Enlargement
Article type: Research Article
Authors: Hicks-Berger, Carrie | Morré, D. James
Affiliations: 1945 Ridgetop Drive, Charlottesville, VA, USA | Department of Medicinal Chemistry and Molecular Pharmacology, HANS Life Sciences Research Building, Purdue University, 201 South University Street, West Lafayette, IN 47907-2064, USA
Note: [] Address for correspondence: D.J. Morré, Department of Medicinal Chemistry and Molecular Pharmacology, HANS Life Sciences Research Building, Purdue University, 201 South University Street, West Lafayette, IN 47907-2064, USA. Fax: +1 765 494 4007; E-mail: morre@pharmacy.purdue.edu
Abstract: The concept that the location of an AAA-ATPase associated with the plant plasma membrane may be indicative of a functional relationship to growth or cell enlargement by analogy with roles in physical membrane displacements as proposed for AAA-ATPases associated with internal membranes was tested. A plant growth hormone-responsive and nucleoside triphosphate-dependent enlargement of inside-out vesicles of plasma membranes from soybeans was utilized in a completely cell-free system. The rate of enlargement was accelerated by the synthetic plant growth factor 2,4-dichlorophenoxyacetic acid (2,4-D) in a log dose-dependent manner and was increased approximately 2-fold with the addition of 1 μM 2,4-D plus 100 μM ATP compared to 100 μM ATP alone, 1 μM 2,4-D alone or no additions. The cell-free enlargement was inhibited by AAA-ATPase-specific antisera and by CoCl_{2}, an inhibitor specific for AAA-ATPases. The responsible ATP site appears to be on the inside of the cell, since right side-out vesicles did not enlarge in response to either ATP, 2,4-D or the two in combination.
Keywords: Cell enlargement, cell-free, ATP, 2,4-dichlorophenoxyacetic acid (2,4-D), auxin, AAA-ATPase, soybean, plant
Journal: BioFactors, vol. 28, no. 2, pp. 91-104, 2006
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