Affiliations: Inflammation and Immunology, Graduate School of
Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan | Molecular Gastroenterology and Hepatology, Graduate
School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto,
Japan | Medical Proteomics, Kyoto Prefectural University of
Medicine, Kyoto, Japan
Note: [] Address for correspondence: Norimasa Yoshida, Molecular
Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto
Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto,
602-8566 Japan. Tel.: +81 75 251 5519; Fax: +81 75 251 0710; E-mail:
nyoshida@koto.kpu-m.ac.jp
Abstract: We investigated the effect of vitamin E on gastric mucosal injury
induced by Helicobacter pylori (H. pylori) infection. Male Mongolian gerbils
were divided into 4 groups (normal group without H. pylori infection, vitamin
E-deficient, -sufficient and -supplemented groups with H. pylori infection).
Following oral inoculation with H. pylori (ATCC43504 2 ×
10^{8} CFU), animals were fed diets α-tocopherol 2
mg/100 g diet in the normal and vitamin E-sufficient groups and
α-tocopherol 0.1 mg/100 g and 50 mg/100 g in the vitamin E-deficient and
-supplemented groups, respectively, for 24 weeks. Chronic gastritis was
detected in all gerbils inoculated H. pylori. Gastric ulcer was detected
in 2 of 7 gerbils only in the vitamin E-deficient group. In the vitamin
E-deficient group, myeloperoxidase activity and mouse keratinocyte derived
chemokine (KC) in gastric mucosa was significantly higher than in the vitamin E
supplemented group. Subsequently, in an in vitro study expression of
CD11b/CD18 on neutrophils was enhanced by H. pylori water extract. This
effect was suppressed in a dose dependent manner by the addition of
α-tocopherol. These results suggest that vitamin E has
a protective effect on gastric mucosal injury induced by H. pylori
infection in gerbils, through the inhibition of accumulation of activated
neutrophils.
Keywords: Helicobacter pylori, vitamin E, adhesion molecules, gastritis
Journal: BioFactors, vol. 28, no. 1, pp. 9-19, 2006
