Affiliations: Pharmaceutical Research Laboratories, Shiseido
Research Center, Yokohama, Japan | Functional Food Research Laboratories, Shiseido
Research Center, Yokohama, Japan | Mitsubishi Kagaku Bio-Clinical Laboratories, Inc.,
Tokyo, Japan
Note: [] Address for correspondence: Yutaka Ashida, Pharmaceutical
Research Laboratories, Shiseido Research Center, Yokohama, 236-8643, Japan.
Tel.: +81 45 788 7263; Fax: +81 45 788 7271; E-mail:
yutaka.ashida@to.shiseido.co.jp
Abstract: We have already shown that prolonged supplementation of
CoQ_{10} in humans reduces the wrinkle area rate and wrinkle
volume per unit area in the corner of the eye. CoQ_{10}
supplementation is known to increase the CoQ_{10} level in
serum and in many organs; however, the level of CoQ_{10} in
skin has not yet been fully investigated yet. We examined whether
CoQ_{10} intake elevates the CoQ_{10} and
CoQ_{9} levels in epidermis, dermis, serum and other organs
(kidney, heart, brain, muscle and crystalline lens) in 43-week-old hairless
male mice. We also established a method using a high performance liquid
chromatograph equipped with an electrochemical detector (HPLC-ECD) to
simultaneously quantify CoQ_{9} and
CoQ_{10} in the tissues. CoQ_{10} (0, 1,
100 mg/kg p.o.) was administered daily for 2 weeks. CoQ_{10}
supplementation of 100 mg/kg increased the serum and epidermal
CoQ_{10} levels significantly, but did not increase the
CoQ_{10} levels in either dermis or other organs. In
conclusion, we showed that CoQ_{10} intake elevates the
epidermal CoQ_{10} level, which may be a prerequisite to
the reduction of wrinkles and other benefits related to the potent antioxidant
and energizing effects of CoQ_{10} in skin.
