Affiliations: Institute of Biochemistry, Università
Politecnica delle Marche, Ancona, Italy | Institute of Microbiology and Biomedical Sciences,
Università Politecnica delle Marche, Ancona, Italy | Dental School, Università Politecnica delle
Marche, Ancona, Italy | Institute of Anatomy and Pathologic Histology,
Università Politecnica delle Marche, Ancona, Italy | Department of Periodontology, Dental School,
Universidad de Sevilla, Sevilla, Spain
Note: [] Address for correspondence: Dr. Maurizio Battino, Institute of
Biochemistry, Faculty of Medicine, Università Politecnica delle Marche,
Via Ranieri, 65, 60131 Ancona, Italy. Tel.: +39 071 2204646; Fax: +39 071
2204398; E-mail: m.a.battino@univpm.it
Abstract: Reactive oxygen species and antioxidant status in periodontal
diseases and periodontal-related pathologies is an item of growing interest.
Immunohistochemical approach may be usefully employed in the study of soft
tissues affected by periodontal disease, giving valuable information on tissue
morphology and vascular proliferation that depends directly on the inflammatory
state. In order to study CoQ_{10} and vitamin E content
in healthy gingiva and in gingivitis a new adaptation to previously published
methods for their determination was adopted. During gingivitis tissue displayed a large inflammatory infiltration
in the lamina propria and a VEGF positive squamous epithelium. The inflammatory
infiltration consisted mainly of lymphocytes, plasma cells and neutrophils.
Vitamin E dramatically decreased and CoQ_{10} remained
unchanged despite the increased amount of cells present in the periodontally
affected tissues, indicating that continuous oxidative stress which occurred in
these structure affected the antioxidant pattern of the tissue.
Keywords: VEGF, MVD, oxidative stress, gingivitis
Journal: BioFactors, vol. 25, no. 1-4, pp. 213-217, 2005
