Affiliations: Korea Food Research Institute, San 46-1, Bundang-gu,
Sungnam-si, Kyunggi-do, 463-746, Korea | Graduate School of East-West Medical Science, Kyung
Hee University, Yongin-si, Kyunggi-do, 499-701, Korea
Note: [] Address for correspondence: Inwook Choi, Ph.D., Korea Food
Research Institute, San 46-1, Bundang-gu, Sungnam-si, Kyunggi-do, 463-746,
Korea. Tel.: +82 31 780 9097; Fax: +82 31 709 9876; E-mail: choiw@kfri.re.kr
Abstract: Soluble glucan, which was obtained from action of
glucosyltransferases (GTFs) of Streptococcus mutans on sucrose, was partially
hydrolyzed by acetic acid and examined for human T lymphoblast (MOLT-4)
stimulating activity. Addition of the partially hydrolyzed glucan (15–60
μg/ml) stimulated human T cell (39–65%) in a dose dependant manner
according to MTT assay. Production of interleukine-2 (IL-2) and interleukine-2
receptor (IL-2R) from T cell was increased by 44.5 and 25%, respectively, by
addition of partially hydrolyzed glucan (15μg/ml). These results indicate
that stimulation of human T cells by hydrolyzed glucan is probably caused by
its effects on stimulating gene expression of IL-2 and IL-2R of human T
cell.
Keywords: Glucan, GTFs, S. mutans, T cell
Journal: BioFactors, vol. 26, no. 1, pp. 1-5, 2006
