A novel test for identifying genes involved in aldehyde
detoxification in the yeast. Increased sensitivity of superoxide-deficient
yeast to aldehydes and their metabolic precursors
Issue title: HNE and Further Lipid Peroxidation
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Affiliations: Department of Biochemistry and Cell Biology,
University of Rzeszów, Poland | Department of Biochemistry, Zamość
College of Agriculture, Poland
Note: [] Address for correspondence: Prof. Tomasz Biliński,
Department of Biochemistry and Cell Biology, University of Rzeszów,
Rejtana 16C, 35-959 Rzeszow, Poland. Tel.: +48 17 8721252; Fax: +48 17 8533504;
E-mail: bilinski@univ.rzeszow.pl
Abstract: A novel test for the identification of genes involved in aldehyde
metabolism is proposed, based on detection of altered sensitivity of the yeast
to corresponding alcohols, metabolic precursors of the aldehydes. This attitude
enabled to an unexpected detection increased sensitivity of mutants devoid of
CuZn-superoxide dismutase (CuZnSOD) to allyl alcohol (precursor of acrolein)
and nonenol. We interpret this finding as due to inactivation of some important
element of aldehyde detoxification by increased flux of superoxide in
ΔCuZnSOD mutants.
