Affiliations: Federal Research Centre for Nutrition and Food,
Institute of Dairy Chemistry and Technology, P.O. Box 60 69, D-24121 Kiel,
Germany. Tel.: +49 431 609 2265; Fax: +49 431 609 2300; E-mail:
hartmann@bafm.de
Note: [] Corresponding author
Abstract: In the context of the EU research project FAIR-CT 98-3077, studies
were carried out to investigate caseinophosphopeptides (CPP) as potential
ingredients for functional food and pharmaceutical applications. CPP preparations were produced by enrichment of CPP from hydrolytic
casein digests. Enzyme preparations used for hydrolysis were PTN 3.0 S,
Alcalase, Bioprotease P conc, Proteinase DS. Cytochemical studies were carried
out to examine the cytotoxic potential or cell modulating activities of CPP
using human cancer cell lines (HL-60, Caco-2) and non-malignant
polymorphonuclear leukocytes (PML) from oral cavity. PML cells isolated by
magnetic cell sorting using CD-15-antibody-labelled paramagnetic beads were
used for the first time for testing food-derived peptides. Cell activity was
measured by formazan dye formation. Effects on enterocytic differentiation
properties of Caco-2 cells were examined by transepithelial membrane resistance
of Caco-2 cell monolayers and brush border associated alkaline phosphatase
activity. In conclusion, (1) no deleterious cytochemical consequences
(apoptotic, antiproliferative or general cytotoxic effects) were observed on
presenting a range of CPP preparations to various human cell systems,
indicating that these compounds can be rated harmless at a cellular level, (2)
stimulation of IgG-secretion into culture supernatant of PBL points to possible
immunoenhancing properties of CPP preparations.
