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Issue title: The Proceedings of the 3rd International Conference on Food Factors (ICoFF 03)
Article type: Research Article
Authors: Lee, Eun-Jung | Jang, Hae-Dong
Affiliations: Department of Food and Nutrition, Hannam University, Daejeon, Korea
Note: [] Address for correspondence: Hae-Dong Jang, Department of Food and Nutrition, Hannam University, 133 Ojungdong Daedukgu, Daejeon, Korea. Tel.: +82 42 629 7393: Fax: +82 42 636 0268; E-mail: haedong@hannam.ac.kr
Abstract: Rooibos tea (Aspalathus linearis) was extracted by refluxing with water and 75% ethanol as a solvent. Antioxidant activity and protective effect on DNA strand scission were investigated by using different antioxidant assay systems and DNA strand nicking assay, respectively. 75% Ethanol extract has higher content of total soluble phenolics and flavonoid than water extract. Antioxidant activities such as hydrogen donating capacity and scavenging activity of hydrogen peroxide were higher in 75% ethanol extract than in water extract except the rate constant with hydroxyl radical. Peroxyl radical induced DNA strand scission was prevented by both 75% ethanol and water extract and hydroxyl radical induced DNA strand scission was not. This result indicates that total soluble phenolics, specially flavonoid, of Rooibos tea are responsible for several kinds of antioxidant activities and preventive activity on peroxyl radical induced DNA strand scission.
Keywords: rooibos tea, total soluble phenolics, antioxidant activity, protective effect on DNA strand scission
Journal: BioFactors, vol. 21, no. 1-4, pp. 285-292, 2004
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