Affiliations: Department of Food Science and Technology, School of
Agricultural Biotechnology, Seoul National University, Seoul 151-744, South
Korea | Division of Food Science, College of Life and
Environmental Sciences, Korea University, Seoul 136-701, South Korea
Note: [] Address for correspondence: Professor Hyong Joo Lee Department
of Food Science and Technology, Seoul National University, Shinlim-dong,
Kwanak-ku, Seoul 151-742, South Korea. Tel.: +82 2 880 4853; Fax: +82 2 873
5095; E-mail: leehyjo@snu.ac.kr
Abstract: Peptide fractions showing anticancer activity were isolated from
anchovy sauce, and their abilities to induce apoptosis in a human lymphoma cell
(U937) were determined by biochemical and flow cytometric methods. The butanol
extract (fraction Aob) from the desalted hydrophobic peptide fraction (Ao)
separated from anchovy sauce by HP-20 adsorption chromatography was found to
possess strong antiproliferative activity against U937 by inducing apoptosis.
Treatment of U937 with Aob resulted in sub-G1 peak representing apoptosis in
the cell cycle analysis, and apoptotic DNA fragmentation. Antiproliferative
activity of the peptide fraction further purified successively by silica gel
chromatography, TLC, and reversed-phase HPLC increased proportionally with the
purification fold. The peptide fraction having antiproliferative activity was
found to be composed of Ala and Phe, and its molecular weight was estimated to
be 440.9 Da.
