Affiliations: School of Humanities, Environmental Policy and
Technology, Himeji Institute of Technology, Japan | Department of Nutrition, Dietetics and Food Science,
Curtin University of Technology, Perth, WA, Australia
Note: [] Address for correspondence: Naoko Hiramatsu, University of
Hyogo, 1--12 1-chome Shinzaike-honcho, Hyogo 670-0092, Japan. Tel.: +81 792 92
1515; Fax: +81 792 93 5710; E-mail: hiramats@shse.u-hyogo.ac.jp
Abstract: Epidemiological studies suggest that the red wine consumption may
reduce the risk factor of cardiovascular disease. However, the mechanisms of
how the red wine phenolic components reduce the risk of cardiovascular disease
is currently unknown. Our previous study demonstrated that red wine
polyphenolics suppress the secretion of pro-atherogenic lipoproteins (very low
density lipoproteins) from human hepatic HepG2 cells. Therefore, in this study
we hypothesize that red wine polyphenolics will also attenuate the production
and secretion of another pro-atherogenic lipoprotein (chylomicrons) from human
intestinal CaCo-2 cells. Cultured CaCo-2 cells were incubated in the presence
of dealcoholized red wine, alcoholized red wine and atorvastatin for 24 h. The
apo B48 protein (marker of intestinal chylomicrons) was quantified on Western
blotting and the enhanced chemiluminescence. Apo B48 levels in the cells and
that secreted into the media were significantly reduced by 29% in the cells
incubated with dealcoholized red wine compared with control cells. Also the
similar effect was shown in the cells incubated with alcoholized red wine. The
cells incubated with atorvastatin shown the significant reduction of apo B48
production compared to control cells. Collectively, this study suggests that
red wine polyphenolics down regulate the production of chylomicron in
intestinal CaCo-2 cells.
Keywords: Caco-2, apoB48, chylomicron, red wine polyphenolics, atherosclerosis
Journal: BioFactors, vol. 22, no. 1-4, pp. 181-183, 2004
