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Issue title: The Proceedings of the 3rd International Conference on Food Factors (ICoFF 03)
Article type: Research Article
Authors: Park, Yoo Kyoung | Lee, Hyang Burm; | Jeon, Eun-Jae | Jung, Hack Sung | Kang, Myung-Hee
Affiliations: Department of Medical Nutrition, Kyunghee University, 1 Hoekidong, Dongdaemoonku, Seoul 130-701, South Korea | Department of Food and Nutrition, Hannam University, 133 Ojeong-dong, Daedeok-gu, Daejeon 306-791, Korea | School of Biological Sciences, Seoul National University, Kwanak-gu, Seoul 151-742, Korea | Seson Biotech, Daejeon 305-710, Korea
Note: [] Corresponding author. Tel.: +82 42 629 7491; E-mail: mhkang@hannam.ac.kr
Abstract: The Chaga mushroom (Inonotus obliquus) is claimed to have beneficial properties for human health, such as anti-bacterial, anti-allergic, anti-inflammatory and antioxidant activities. The antioxidant effects of the mushroom may be partly explained by protection of cell components against free radicals. We evaluated the effect of aqueous Chaga mushroom extracts for their potential for protecting against oxidative damage to DNA in human lymphocytes. Cells were pretreated with various concentrations (10, 50, 100 and 500 μg/mL) of the extract for 1 h at 37°C. Cells were then treated with 100 μM of H_2O_2 for 5~min as an oxidative stress. Evaluation of oxidative damage was performed using single-cell gel electrophoresis for DNA fragmentation (Comet assay). Using image analysis, the degree of DNA damage was evaluated as the DNA tail moment. Cells pretreated with Chaga extract showed over 40% reduction in DNA fragmentation compared with the positive control (100 μmol H_2O_2 treatment). Thus, Chaga mushroom treatment affords cellular protection against endogenous DNA damage produced by H_2O_2.
Keywords: chaga extract, comet assay, human lymphocyte, Inonotus obliquus, oxidative DNA damage
Journal: BioFactors, vol. 21, no. 1-4, pp. 109-112, 2004
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