High-throughput profiling of dietary polyphenols and their metabolites by HPLC-ESI-MS-MS in human urine

Issue title: The Proceedings of the 3rd International Conference on Food Factors (ICoFF 03)

Article type: Research Article

Authors: Ito, Hideyuki | Gonthiera, Marie-Paule | Manach, Claudine | Morand, Christine | Mennen, Louise | Rémésy, Christian | Scalbert, Augustin

Affiliations: Unité des Maladies Métaboliques et Micronutrients, INRA, 63122 Saint-Genès-Champanelle, France | Unité de Surveillance et d'Epidémiologie Nutritionnelle, ISTNA, Paris, France

Note: [] Corresponding author. Present address: Hideyuki Ito, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama 700-8530, Japan. E-mail: hito@cc.okayama-u.ac.jp

Abstract: Polyphenols in biological fluids are generally estimated by HPLC with UV, electrochemical or fluorimetric detection. We describe herein a method specially developed to estimate biomarkers of polyphenol consumption in human urine. We simultaneously quantified 15 polyphenols and their related compounds in human urine using an HPLC coupled with electrospray ionization mass-mass (HPLC-ESI-MS-MS) method with an analytical run time of 6 min. The method has been validated with respect to linearity, precision, and accuracy in intra- and inter-day assays. It has been applied to human urine samples collected from volunteers after consumption of different polyphenol-rich beverages in controlled conditions.

Keywords: HPLC-ESI-MS-MS, polyphenols, human urine, metabolites, biomarkers

Journal: BioFactors, vol. 22, no. 1-4, pp. 241-243, 2004