Benzyl isothiocyanate inhibits oxidative stress in mouse skin: Involvement of attenuation of leukocyte infiltration

Issue title: The Proceedings of the 3rd International Conference on Food Factors (ICoFF 03)

Article type: Research Article

Authors: Nakamura, Yoshimasa | Miyoshi, Noriyuki | Takabayashi, Satoko | Osawa, Toshihiko

Affiliations: Laboratory of Food and Biodynamics, Nagoya University Graduate School of Bioagricultural Sciences, Nagoya 464-8601, Japan. Tel.: +81 52 789 4126; Fax: +81 52 789 5296; E-mail: yossan@agr.nagoya-u.ac.jp

Note: [] Corresponding author

Abstract: The exposure of benzyl isothiocyanate (BITC) to mouse skin resulted in the attenuation of 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced oxidative damage through not only inhibition of the NADPH oxidase system but also leukocyte clearance at inflamed region. In spite of little ability to affect TPA-induced edema formation, pretreatments of mouse skin with BITC before the first or second TPA treatment significantly decrease the H_2O_2 level. A histological study also demonstrated that BITC enhanced the terminal deoxynucleotidyl transferase-dUTP nick end labeling (TUNEL)-positive index in mouse skin, suggesting that BITC might accelerate the disappearance of infiltrated leukocytes. Thus, these gathered data further supported that BITC has a potential as an anti-inflammatory agent.

Keywords: benzyl isothiocyanate, tumor promotion, reactive oxygen species, neutrophil, NADPH oxidase

Journal: BioFactors, vol. 21, no. 1-4, pp. 255-257, 2004