Transbilayer distribution of aminophospholipids and the oxidative stability of their component polyunsaturated fatty acids

Issue title: The Proceedings of the 3rd International Conference on Food Factors (ICoFF 03)

Article type: Research Article

Authors: Kubo, Kazuhiro | Sekine, Seiji | Saito, Morio

Affiliations: Division of Food Science, Department of Food Science Research for Health, Incorporated Administrative Agency, National Institute of Health and Nutrition, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8636, Japan

Note: [] Corresponding author. Tel.: +81 3 3203 5601; Fax: +81 3 3203 7584; E-mail: kkubo@nih.go.jp

Abstract: We examined the relationship between the transbilayer distribution of aminophospholipids, such as phosphatidylethanolamine (PE), PE plasmalogen and phosphatidylserine, and the oxidative stability of polyunsaturated fatty acids (PUFAs) in the aminophospholipids. To modulate the transbilayer distribution of aminophospholipid in liposomes, we used phosphatidylcholine (PC) with two types of acyl chain region: dipalmitoyl (PC16:0) or dioleoyl (PC18:1). In the smaller-sized liposomes, the proportions of aminophospholipid in the liposomal external layer were significantly higher in liposomes containing PC18:1 than in those containing PC16:0. Additionally, aminophospholipids in the external layer of smaller-sized liposomes were able to protect their component PUFAs from 2,2'-azobis(2-amidinopropane)dihydrochloride-mediated lipid peroxidation.

Keywords: acyl chain region, aminophospholipid, asymmetric distribution, lipid peroxidation, liposome, phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, plasmalogen, transbilayer distribution

Journal: BioFactors, vol. 21, no. 1-4, pp. 235-239, 2004