Affiliations: The Department of Applied Bioscience, Tokyo University
of Agriculture, 1-1-1, Sakuragaoka Setagaya, Tokyo, 156-8502, Japan. Tel.: +81
3 5477 2453; Fax: +81 3 5477 7222; E-mail: swatanab@nodai.ac.jp | The Japan Institute for the Control of Aging,
Shizuoka, Japan
Note: [] Corresponding author
Abstract: Little is known about the direct effect of broccoli sprouts on human
health. So we investigated the effect of broccoli sprouts on the induction of
various biochemical oxidative stress markers. Twelve healthy subjects (6 males
and 6 females) consumed fresh broccoli sprouts (100 g/day) for 1 week for a
phase 1 study. Before and after the treatment, biochemical examination was
conducted and natural killer cell activity, plasma amino acids, plasma PCOOH
(phosphatidylcholine hydroperoxide), the serum coenzyme
Q_{10}, urinary 8-isoprostane, and urinary 8-OHdG
(8-hydroxydeoxyguanosine) were measured. With treatment, total cholesterol and
LDL cholesterol decreased, and HDL cholesterol increased significantly. Plasma
cystine decreased significantly. All subjects showed reduced PCOOH,
8-isoprostane and 8-OHdG, and increased
CoQ_{10}H_{2}/CoQ_{10}
ratio. Only one week intake of broccoli sprouts improved cholesterol metabolism
and decreased oxidative stress markers.
