Antitumor protein (AP) from a mushroom induced apoptosis to transformed human keratinocyte by controlling the status of pRb, c-MYC, cyclin E-cdk2, and p21^{\rm WAF1} in the G1/S transition

Article type: Research Article

Authors: Yukio Kawamura, | Mariko Manabe, | Kazumi Kitta,

Affiliations: National Food Research Institute, 2-1-2 Kannon-dai, Tsukuba, Ibaraki 305-8642, Japan

Abstract: Antitumor protein (AP) from a mushroom, induced the morphological changes typical to apoptosis such as nuclear condensation, aneuploidity, and DNA fragmentation at concentrations as low as 5--20 ng/ml to cancer cells. Molecular alterations related to cell cycle. Molecular alterations related to cell cycle, especially G1/S transition were investigated with a human keratinocyte transformed with oncoproteins, E6 and E7 of human pappiloma virus(HPV)-16. AP didn't alter significantly and oncosuppressor p53 level, but induced hyperphosphorylation of pRb. Time-dependent change of G1 cyclins, cdk2 and cdk4 after addition of AP showed that expression level of cdk inhibitors, INK4 family, and p27^{\rm KIP1} did not altered, while that of p21^{\rm WAF1} was downregulated.

Journal: BioFactors, vol. 12, no. 1-4, pp. 157-160, 2000