Rapid and Sensitive Controlled Release Monitoring Method of Biomedical Combined Products with IDM for Pain Management and Cancer Treatment
Issue title: Frontiers in Biomedical Engineering and Biotechnology – Proceedings of the 2nd International Conference on Biomedical Engineering and Biotechnology, 11–13 October 2013, Wuhan, China
Affiliations: Department of Life Science, College of Science and Engineering, Fu Jen Catholic University, No. 510, Zhongzheng Rd., Xinzhuang Dist., New Taipei City 24205, Taiwan | Department of Biomedical Engineering, Ming-Chuan University, 5 De Ming Rd., Gui Shan District, Taoyuan, 333, Taiwan | Metal Industries Research & Development Centre, 6F, No.162-24, Sec.3, Hsin-Yi Rd., Taipei 10658, Taiwan
Abstract: New designed combined products and its determination for pain management and cancer treatment were studied. A rapid and sensitive stability indicating HPLC method had been developed and validated for the determination of Indomethacin(IDM) in a transdermal patch. This analytical method was successfully applied to the determination of Indomethacin in a transdermal patch and can be used for routine quality control analysis. Chromatographic separation was achieved isocratically on an Inertsil® C8-3 column utilizing a mobile phase of acetonitrile / 0.01 M monobasic sodium phosphate and 0.01M dibasic sodium phosphate buffer (pH 3) (65:35, v/v) at the flow rate of 1 mL/min with UV detection at the wavelength of 210 nm. The system suitability was performed, and the result showed that Indomethacin(IDM) and its impurity were separated. The calibration curve of Indomethacin(IDM) was linear in the range of 0.1~15 ppm (r = 0.9989, n = 3).
Keywords: Controlled release, HPLC, Indomethacin, transdermal route, drug delivery
