Biological characteristics and oxidation mechanism of a new manganese-oxidizing bacteria FM-2
Issue title: Frontiers in Biomedical Engineering and Biotechnology – Proceedings of the 2nd International Conference on Biomedical Engineering and Biotechnology, 11–13 October 2013, Wuhan, China
Affiliations: Department of Chemistry, Tongji University, Shanghai, 200092, China | School of Life Sciences and Technology, Tongji University, Shanghai, 200092, China
Note: [] Address for correspondence: Wenwei Tang, Room 437, Department of Chemistry, Tongji University, #1239 Siping Rd, Shanghai, China. Tel.: +86 21 65983366; fax: +86 21 65983366; E-mail:tangww@tongji.edu.cn.
Abstract: A new manganese-oxidizing strain FM-2 was screened out from biological activated carbon (BAC) filter column and was identified as Citrobacter freundii. The results of the systematic study on this species are as follows: At 27°C, the optimum pH for Citrobacter sp. FM-2 to remove manganese was 7.0–8.0.The best removal rate of manganese under 27°C, pH 7.0 by FM-2 was reached at 4 d, being 76.2%; Compared with adsorption, biological oxidation played a dominant role in this removing process. Almost 75.7% of manganese was oxidized into oxides by Citrobacter sp and there were some particular oxides analogs generated on the bacterial surface; A 296bp DNA fragment amplified from Citrobacter sp. FM-2 revealed that this species has multicopper oxidase genes. Meanwhile, the phylogenetic tree indicated that compared with other related species, Citrobacter sp. FM-2 has its own evolutional independence.
