Effect of Nd:YAG laser-nitriding-treated titanium nitride surface over Ti6Al4V substrate on the activity of MC3T3-E1 cells

Issue title: Frontiers in Biomedical Engineering and Biotechnology – Proceedings of the 2nd International Conference on Biomedical Engineering and Biotechnology, 11–13 October 2013, Wuhan, China

Article type: Research Article

Authors: Wang, Min^{; ;} | Ning, Yingyuan^{; ;} | Zou, Haixiao^; | Chen, Si^; | Bai, Yi^; | Wang, Aihua | Xia, Haibin^{; ;}

Affiliations: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, Wuhan University, Wuhan, China | Department of Oral Implantology, School & Hospital of Stomatology, Wuhan University, Wuhan, China | Department of stomatology, Armed Police Border Defense Corps Hospital of GuangDong province | Department of Oral and Maxillofacial Surgery, School & Hospital of Stomatology, Wuhan University, Wuhan, China | Department of Materials Science & Engineering, Huazhong University of Science and Technology, Wuhan, China

Note: [] These authors contributed equally to this work.

Note: [] These authors contributed equally to this work.

Note: [] Address for correspondence: Haibin Xia, Department of Oral Implantology, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, P.R. China. Tel: +86 27 87686222, Fax: +86 27 87873260. E-mail: xhaibin@whu.edu.cn

Abstract: Ti6Al4V discs with a thickness of 2.5 mm and dimensions of 15 × 15 mm2 were fabricated. The titanium nitride (TiN) surface was formed via Nd:YAG laser-nitriding. A sandblast acid-etched (SA) surface was used as a control. Scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), and surface roughness tests were conducted to study the surface and cross-section morphologies as well as the properties of TiN and SA surfaces. MC3T3-E1 osteoblast-like cells were cultured on the TiN and SA surfaces to evaluate the effect of TiN surface on cellular behaviors, including attachment, proliferation and differentiation. Morphological testing results revealed that the cross-section of TiN exhibited dendritic crystallization without cracking. The proliferation and differentiation of MC3T3-E1 cells on the laser-nitriding TiN surface were significantly increased compared to those cultured on SA surface. These findings suggested that the TiN surface generated from Nd:YAG laser-nitriding were favorable for the proliferation and differentiation of MC3T3-E1 cells, which is significant for implant surface modification.

Keywords: laser-nitriding, TiN surface, MC3T3-E1 cells, adhesion, differentiation

DOI: 10.3233/BME-130852

Journal: Bio-Medical Materials and Engineering, vol. 24, no. 1, pp. 643-649, 2014