Affiliations: CNRS, UMR 7561 et FR3209, Plate Forme d'Imagerie Cellulaire et Tissulaire PTIBC-IBISA, Nancy-Université, Faculté de Médecine, Vandoeuvre-lès-Nancy, France | CNRS, UMR 7561 et FR3209, Physiopathologie, Pharmacologie et Ingénierie Articulaires UMR 7561 CNRS-UHP et Ingénierie Moléculaire et Thérapeutique FR CNRS-INSERM 3209, Nancy-Université, Faculté de Médecine, Vandoeuvre-lès-Nancy, France | Plate-forme “Microscopie–Imagerie–Cytométrie du campus Pasteur Lille, IFR 142 Institut Pasteur de Lille, Institut de Biologie de Lille, Lille, France | Nancy Universités, Nancy, France
Note: [] Address for correspondence: D. Dumas, CNRS, UMR 7561 et FR3209, Plate Forme d'Imagerie Cellulaire et Tissulaire PTIBC-IBISA, Nancy-Université, Faculté de Médecine, 54505 Vandoeuvre-lès-Nancy Cedex, France. E-mail: dominique.dumas@medecine.uhp-nancy.fr.
Abstract: For this study, we have considered a new large field of view imaging dedicated to matrix collagen (no stained samples). To integrate a multidimensional scale (non-sliced samples), a femtosecond oscillator (two photon excitation laser) has been coupled with a large field optical setup to collect SHG signal. We introduced an index (F–SHG) based on decay time response measured by TCSPC for, respectively, Fluorescence (F) and Second Harmonic Generation (SHG) values. For samples where protein collagen is the major component of extracellular matrix (skin) or not (nacre), we compared the index distribution (from 2 to 12) obtained with large field optical setup. In this work, we showed for the first time that multiscale large field imaging combined to multimodality approaches (SHG-TCSPC) could be an innovative and non invasive technique to detect and identify some biological interest molecules (collagen) in biomedical topics.
Keywords: Multiphoton, second harmonic generation, collagen, extracellular matrix
