Affiliations: Department of Social Dentistry, Matsumoto Dental University, Nagano, Japan | Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University, Tokyo, Japan | Department of Dental Education (Department of Oral Anatomy), School of Dentistry, Showa University, Tokyo, Japan | Department of Biomedical Sciences, College of life Sciences, Ritsumeikan University, Kusatsu, Japan | Division of oral health promotion, Institute for oral science, Matsumoto Dental university, Nagano, Japan | Department of Biomaterials, School of Dentistry, Birmingham University, Birmingham, UK | Professor Emeritus, Hokkaido University, Sapporo, Japan
Note: [] Address for correspondence: Kimitoshi Yagami, Department of Social Dentistry, Matsumoto Dental University, 1780 Hirohoka Gohobara, Shiojiri, Nagano 399-0781, Japan. Tel.: +81 0263 51 2153; Fax: +81 0263 51 2153; E-mail: kyagami@po.mdu.ac.jp.
Abstract: To establish an effective method for bone augmentation, we introduced a new honeycomb-like β-tricalcium phosphate (H-β-TCP) with BMP-2 as a scaffold, whose unique geometrical properties induce osteoblastic differentiation of autologous bone marrow mesenchymal stem cells (BMSCs). A total of six beagle dogs from 6 to 7 years old were used for this study. BMSCs were cultured with autologous serum and BMP-2 on H-β-TCP. Differentiation to osteoblasts was demonstrated in vitro and exo vivo. Scanning electron microscopy revealed formation and calcification of a matrix-like structure within the H-β-TCP tunnels in BMSC culture. Moreover, treatment of BMP-2 promoted osteoblastic differentiation of BMSCs in H-β-TCP in a diffusion chamber. These results indicated that H-β-TCP may be a useful tool for construction of functional artificial bone.
Keywords: Honeycomb-form β-TCP, osteoblastic differentiation, bone marrow mesenchymal stem cells
