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Article type: Research Article
Authors: Hashimoto, Yoshiya; | Adachi, Seita | Matsuno, Tomonori | Omata, Kazuhiko | Yoshitaka, Yamauchi | Ozeki, Yasuyuki | Umezu, Yoshikazu | Satoh, Tazuko | Nakamura, Masaaki
Affiliations: Department of Biomaterials, Osaka Dental University, Hirakata, Osaka, Japan | Department of Oral and Maxillofacial Surgery, The Nippon Dental University, School of Life Dentistry at Tokyo, Tokyo, Japan | New Material Science Laboratory, Advance Co., Tokorozawa, Saitama, Japan
Note: [] Address for correspondence: Yoshiya Hashimoto, Department of Biomaterials, Osaka Dental University, Osaka, 8-1 Kuzuhahanazono-cho, Hirakata, Osaka 573-1121, Japan. Tel.: +81 72 864 3056; Fax: +81 72 864 3156; E-mail: yoshiya@cc.osaka-dent.ac.jp.
Abstract: The objective of this study was to evaluate the effect of β-tricalcium phosphate (β-TCP) bead size on the behavior of KUSA/A1 mouse osteoblasts when the β-TCP beads are used as the solid phase of a scaffold in which alginate was used as the gel phase. KUSA/A1 cells were loaded onto a three-dimensional (3D) scaffold fabricated from β-TCP beads with diameters ranging from 300 to 500 μm (small beads), 500–700 μm (medium beads) and 700–850 μm (large beads); cells were cultured for 3, 7 and 14 days. Scanning electron microscope observations showed that each bead was connected in a network consisting of the alginate gel and KUSA/A1 cellular matrix that was tightly bonded to form a 3D structure. After 3 days, cells in the 3D scaffold with medium beads had a significantly higher alkaline phosphatase activity (ALP) than cells in the other scaffolds. However, by 7 and 14 days in culture there was no significant difference in DNA levels, ALP activity or osteocalcin expression. At 8 weeks, only the composite containing small beads and KUSA/A1 cells had turned completely into bone in vivo. Thus, bead size may influence the success of bone formation in this context.
Keywords: β-tricalcium phosphate (β-TCP), alginate, injectable scaffold, bone tissue engineering, 3D scaffold
DOI: 10.3233/BME-2009-0604
Journal: Bio-Medical Materials and Engineering, vol. 19, no. 6, pp. 391-400, 2009
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