Searching for just a few words should be enough to get started. If you need to make more complex queries, use the tips below to guide you.
Issue title: Cell Therapy, Bioengineering and Regenerative Medicine, September 2008, Nancy, France
Article type: Research Article
Authors: Decot, V.; ; | Houzé, P. | Stoltz, J.-F.; | Bensoussan, D.;
Affiliations: CHU de Nancy, Cell Therapy and Tissues Unit, Brabois, Vandoeuvre-les-Nancy, France | Nancy Université UHP, CNRS – Groupe Ingéniérie Cellulaire et Tissulaire – UMR CNRS Faculté de Médecine, Vandoeuvre-les-Nancy, France | APHP, Biochemistry Laboratory, Hospital Saint Louis, Paris, France
Note: [] Address for correspondence: V. Decot, CHU de Nancy, Cell Therapy and Tissues Unit, allée du Morvan, Brabois, 54511 Vandoeuvre-les-Nancy, France. E-mail: v.decot@chu-nancy.fr.
Abstract: Dimethylsulfoxide (DMSO) is a cryoprotective substance often used to allow long term storage of stem cells or tissue grafts. However, a high frequency of adverse events is associated with the infusion of thawed cells. These events are in part due to DMSO, leading many cell therapy facilities to introduce a washing step before the delivery of the grafts. The lack of method for evaluating the residual quantities of this substance in the reinfused cells led us to develop a technique, based on capillary zone electrophoresis for assaying DMSO. The cryoprotectant was measured in 55 hematopoietic stem cell grafts, 6 parathyroids and 5 blood vessels immediately after thawing and after washing or bathing in a saline solution. The results showed that DMSO reduction in stem cell grafts reached more than 90% after the washing procedure. Furthermore, this study has shown that 2 washing steps significantly improved DMSO elimination as compared to 1 washing step. For parathyroids and blood vessels, bathing the tissues after thawing in a saline solution allowed more than 95% DMSO reduction. This study demonstrated that the technique of DMSO measurement used here, is simple and feasible on complex matrices such as protein samples after dilution. It is an appropriate method for residual quantification of the cryoprotectant before graft.
Keywords: DMSO, stem cells cryopreservation, tissue grafts, capillary zone electrophoresis
DOI: 10.3233/BME-2009-0594
Journal: Bio-Medical Materials and Engineering, vol. 19, no. 4-5, pp. 293-300, 2009
IOS Press, Inc.
6751 Tepper Drive
Clifton, VA 20124
USA
Tel: +1 703 830 6300
Fax: +1 703 830 2300
sales@iospress.com
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
IOS Press
Nieuwe Hemweg 6B
1013 BG Amsterdam
The Netherlands
Tel: +31 20 688 3355
Fax: +31 20 687 0091
info@iospress.nl
For editorial issues, permissions, book requests, submissions and proceedings, contact the Amsterdam office info@iospress.nl
Inspirees International (China Office)
Ciyunsi Beili 207(CapitaLand), Bld 1, 7-901
100025, Beijing
China
Free service line: 400 661 8717
Fax: +86 10 8446 7947
china@iospress.cn
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
如果您在出版方面需要帮助或有任何建, 件至: editorial@iospress.nl