Medpor^® regulates osteoblast's microRNAs

Article type: Research Article

Authors: Palmieri, Annalisa | Pezzetti, Furio | Brunelli, Giorgio | Martinelli, Marcella | Scapoli, Luca | Arlotti, Marzia | Masiero, Elena | Carinci, Francesco^;

Affiliations: Institute of Histology, University of Bologna and Center of Molecular Genetics, CARISBO Foundation, Bologna, Italy | Department of DMCCC, Section of Maxillofacial Surgery, University of Ferrara, Ferrara, Italy

Note: [] Address for correspondence: Francesco Carinci, MD, Department of DMCCC, Section of Maxillofacial Surgery, University of Ferrara, Corso Giovecca, 203, 44100 Ferrara, Italy. Tel./Fax: +39 0532 291582; E-mail: crc@unife.it; URL: www.carinci.org.

Abstract: Porous polyethylene (PP or Medpor®) is an alloplastic material worldwide used for craniofacial reconstruction. Although several clinical studies are available, there is a lack as regard the genetic effects. Because PP is always fixed on bone and the mechanism by which PP acts on osteoblasts is unknown, we therefore attempted to address this question by using microRNA microarray techniques to investigate the translation regulation in osteoblasts exposed to PP. The miRNA oligonucleotide microarray provides a novel method to carry out genome-wide microRNA profiling in human samples. By using miRNA microarrays containing 329 probe designed from Human miRNA sequence, we identified in osteoblast-like cells line (MG-63) cultured with Medpor® (Porex Corporation, Fairburn, Georgia, USA) several miRNA which expression is significantly modified. We identified 16 up-regulated miRNA (i.e. mir-337, mir-515-3p, mir-377, mir-153, mir-367, mir-152, let-7b, mir-92, mir-155, mir-424, mir-148b, mir-368, mir-18b, mir-520d, mir-20b, mir-128a) and 2 down-regulated miRNA (i.e. mir-143, mir-32). The data reported are, to our knowledge, the first study on translation regulation in osteoblasts exposed to PP. They can be relevant to better understand the molecular mechanism of bone regeneration and as a model for comparing other materials with similar clinical effects.

Keywords: Alloplastic material, porous polyethylene, miRNA, microarray, gene expression

DOI: 10.3233/BME-2008-0512

Journal: Bio-Medical Materials and Engineering, vol. 18, no. 2, pp. 91-97, 2008