Searching for just a few words should be enough to get started. If you need to make more complex queries, use the tips below to guide you.
Article type: Research Article
Authors: Forest, P.; | Morfin, F. | Bergeron, E. | Dore, J. | Bensa, S. | Wittmann, C. | Picot, S. | Renaud, F.N.R. | Freney, J. | Gagnieu, C.;
Affiliations: UCBL, Laboratoire des Biomatériaux, Dispositifs Médicaux et Remodelages Matriciels, UMR–MA, Lyon, France | UCBL, Biom'Up, ISPB, Lyon, France | UCBL, Nosoco.tech®, ISPB, Laboratoire de Bactériologie, Lyon, France
Note: [] Address for correspondence: Dr. Christian Gagnieu, Equipe des Biomatériaux, Laboratoire des Biomatériaux, Dispositifs Médicaux et Remodelages Matriciels, 8 av Rockefeller, 69373 Lyon cedex 08, France. Tel./Fax: +33 426688216; E-mail: christian.gagnieu@insa-lyon.fr.
Abstract: In the last few years, regulations for biomolecule production, and especially for extraction and purification of animal molecules such as collagen, have been reinforced to ensure the sanitary safety of the materials. To be authorized to market biomaterials based on collagen, manufacturers now have to prove that at least one step of their process is described in guidelines to inactivate prion, viruses, and bacteria. The present study focuses on the inactivation step performed during the extraction and purification of porcine type I atelocollagen. We chose to determine the reduction factor of a 1 M NaOH step on porcine parvovirus and four bacterial strains inactivation. During the extraction step, we deliberately inoculated the collagen suspension with the different microorganisms tested. Then, 1 M NaOH was added to the suspension for 1 hour at 20°C. We demonstrated that this treatment totally inactivated S. aureus, P. aeruginosa, C. albicans and A. niger which are bacterial strains responsible of severe human pathology. The reduction factors reached more than 4 logs for B. cereus spores and 4 logs for the porcine parvovirus. are encouraging as those two microorganisms are known to be very resistant to inactivation.
Keywords: Collagen, sodium hydroxide, porcine parvovirus, bacteria, inactivation
Journal: Bio-Medical Materials and Engineering, vol. 17, no. 4, pp. 199-208, 2007
IOS Press, Inc.
6751 Tepper Drive
Clifton, VA 20124
USA
Tel: +1 703 830 6300
Fax: +1 703 830 2300
sales@iospress.com
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
IOS Press
Nieuwe Hemweg 6B
1013 BG Amsterdam
The Netherlands
Tel: +31 20 688 3355
Fax: +31 20 687 0091
info@iospress.nl
For editorial issues, permissions, book requests, submissions and proceedings, contact the Amsterdam office info@iospress.nl
Inspirees International (China Office)
Ciyunsi Beili 207(CapitaLand), Bld 1, 7-901
100025, Beijing
China
Free service line: 400 661 8717
Fax: +86 10 8446 7947
china@iospress.cn
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
如果您在出版方面需要帮助或有任何建, 件至: editorial@iospress.nl