Searching for just a few words should be enough to get started. If you need to make more complex queries, use the tips below to guide you.
Issue title: Cell and Tissue Bioengineering and Therapy, Nancy 2005, 10–11 May
Article type: Research Article
Authors: Boura, C.; | Kerdjoudj, H. | Moby, V. | Vautier, D. | Dumas, D. | Schaaf, P. | Voegel, J.-C. | Stoltz, J.-F. | Menu, P.
Affiliations: Laboratoire de Mécanique et Ingénierie Cellulaire et Tissulaire, LEMTA-UMR 7563 (CNRS-UHP-INPL) et IFR 111, Faculté de Médecine, 54505 Vandoeuvre-lès-Nancy, France | INSERM U 595, Faculté de Chirurgie Dentaire, 67085 Strasbourg, France | Institut Charles Sadron (CNRS-ULP), UPR 22 CNRS, 67083 Strasbourg, France
Note: [] Corresponding author: C. Boura, Laboratoire de Mécanique et Ingénierie Cellulaire et Tissulaire, LEMTA-UMR 7563 (CNRS-UHP-INPL) et IFR 111, Faculté de Médecine, 54505 Vandoeuvre-lès-Nancy, France. Tel.: +33 3 83 68 34 74; Fax: +33 3 83 68 34 59; E-mail: cedric.boura@medecine.uhp-nancy.fr.
Abstract: Polyelectrolyte multilayer films were recently investigated to favour attachment of Human Vein Umbilical Endothelial Cells (HUVECs) on non-adhesive surfaces. In this study, we evaluated the initial adhesion of HUVECs after 3 h of seeding on two polyelectrolyte multilayer films ending by poly(D-lysine) (PDL) or poly(allylamine hydrochloride) (PAH). In order to obtain information about initial adhesion of HUVECs, cell morphology as well as the expression of β1 integrins, specific receptors of adhesion, were evaluated after 3 h of seeding on polyelectrolyte multilayer films. The data were also compared to PDL or PAH monolayers (polyelectrolytes terminating the multilayer architecture). The expression of β1 integrins was not different, whatever are the studied surfaces. However, HUVECs spreading on polyelectrolyte multilayer films, in particular on PAH ending film, was more important as compared to polyelectrolyte monolayers or glass. In conclusion, the best initial adhesion conditions of HUVECs on polyelectrolyte films could not be elucidated, moreover the results suggested also that β1 integrins could only play a limited role.
Keywords: Polyelectrolyte multilayers, endothelial cells, cell adhesion, atomic force microscopy, β1 integrins
Journal: Bio-Medical Materials and Engineering, vol. 16, no. 4, pp. S115-S121, 2006
IOS Press, Inc.
6751 Tepper Drive
Clifton, VA 20124
USA
Tel: +1 703 830 6300
Fax: +1 703 830 2300
sales@iospress.com
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
IOS Press
Nieuwe Hemweg 6B
1013 BG Amsterdam
The Netherlands
Tel: +31 20 688 3355
Fax: +31 20 687 0091
info@iospress.nl
For editorial issues, permissions, book requests, submissions and proceedings, contact the Amsterdam office info@iospress.nl
Inspirees International (China Office)
Ciyunsi Beili 207(CapitaLand), Bld 1, 7-901
100025, Beijing
China
Free service line: 400 661 8717
Fax: +86 10 8446 7947
china@iospress.cn
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
如果您在出版方面需要帮助或有任何建, 件至: editorial@iospress.nl