Affiliations: Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China | Laboratory of Neuropathology, Chengde Medical University, Chengde 067080, China
Note: [] Corresponding author: Kuanghu Hu, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, P.R. China. Tel.: +86 10 64888589; Fax: +86 10 64877837; E-mail: hukh@sun5.ibp.ac.cn.
Abstract: This paper introduces a three-dimensional (3D) reconstruction algorithm of the brain stem nuclei based on fast centroid auto-registration. The research is based on methods and theories of computer stereo vision, and by image information processing three-point pattern local search, registration and auto-tracing for the centroids of the brain stem nuclei were accomplished. We adopt two-peak threshold, edge detection and grayscale image enhancement to extract contours of the nuclei's structures. The experimental results obtain the spatial structure information and 3D image of the brain stem nuclei, show spatial relationship between 14 pairs of nuclei, and quantitate morphological parameters of each type of nuclei's 3D structure. This work is significant to neuroanatomy research and clinic applications. Furthermore, a software system named BRAIN.HUK is established.
Keywords: Brain stem nuclei, centroid registration, 3D reconstruction, 3D structure quantification
