In vitro evaluation of BMSCs early proliferation on minocycline-loaded electrospun nanofibers membrane

Article type: Research Article

Authors: Liu, Quan^a; | Ma, Xiao^b; | Pei, Yanchen^c | Cheng, Wendan^b; | Wu, Zhengwei^a;

Affiliations: [a] Institute of Advanced Technology, University of Science and Technology of China, Hefei, China | [b] Department of Orthopedics, The Second Affiliated Hospital of Anhui Medical University, Hefei, China | [c] School of Chemistry, Faculty of Science and Engineering, The University of Manchester, Manchester, UK

Correspondence: [*] Corresponding authors: Wendan Cheng, Department of Orthopedics, The Second Affiliated Hospital of Anhui Medical University, Hefei, China. E-mail: sunyccc@126.com. Zhengwei Wu, Institute of Advanced Technology, University of Science and Technology of China, Hefei, China. E-mail: wuzw@ustc.edu.cn

Note: [†] These authors contributed equally to this work.

Abstract: BACKGROUND:Electrospun nanofibers could simulate the natural extracellular matrix (ECM) of the host bone, while minocycline (MINO) is a broad-spectrum tetracycline antibiotic which has been found to have multiple non-antibiotics biological effects that promotes osteogenesis in vitro and in vivo. OBJECTIVE:The present study aims at constructing a polylactic acid (PLA) electrospun nanofiber membrane loaded with MINO to enhance Bone marrow mesenchymal stem cells (BMSCs) adhesion and proliferation for early clinical treatment. METHODS:The MINO-PLA membrane were characterized by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR) and in vitro drug release study. The antibacterial ability was also investigated. In addition, in vitro cellular proliferation experiment was performed to verify whether the PLA electrospun nanofibers membrane loaded with MINO enhance BMSCs adhesion and proliferation. RESULTS:Analyzing the drug release and cell growth results, it was found that only the effective concentration of MINO-PLA could help the growth of BMSCs in the short term. This is related to the drug release rate of MINO-PLA and the initial concentration of MINO. CONCLUSION:This study shows that by controlling the concentration and release rate of MINO with electrospinning PLA, BMSCs could proliferate on it, and a new bone repair material had been made in this study.

Keywords: Minocycline, osteogenesis, electrospun, bone marrow mesenchymal stem cells

DOI: 10.3233/BME-230002

Journal: Bio-Medical Materials and Engineering, vol. 35, no. 1, pp. 1-12, 2024