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Article type: Research Article
Authors: Kumari, T.V.; | Anil Kumar, P.R. | Muraleedharan, C.V. | Bhuvaneshwar, G.S. | Sampeur, Y. | Derangere, F. | Suryanarayanan, R.
Affiliations: BMT Wing, Sree Chitra Tirunal Institute of Medical Sciences & Technology, Poojapura, Thiruvananthapuram, Kerala 695 012, India | ICC, Rue Robert Schuman, Z. I. des Uselles, 77350 Le Mée sur Seine, France | Laboratoire de Physico‐Chimie de l'Etat Solide, UMR 8648, Bât. 414 Université Paris‐Sud, 91405 Orsay, France
Note: [] Corresponding author: Dr. T.V. Kumari, Tissue Culture Laboratory, Division of Implant Biology, Bio Medical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojappura, Thiruvananthapuram‐695 012, India. Tel.: 91 0471 340801; Fax: +91 471 341814; E‐mail: tvkumari@sctimst.ker.nic.in
Abstract: Diamond like carbon (DLC) films were deposited on to titanium (Ti) substrates by Plasma Enhanced Chemical Vapour Deposition (PECVD) process. The quality of the films were checked by Raman spectra and nano‐hardness tests. The cytocompatibility of titanium and DLC coated titanium were studied using continuous cell lines of mouse fibroblast cells ( L‐929), Human Osteoblast cells (HOS) and primary human umbilical cord vein endothelial cells (HUVEC). The cellular responses to the materials were assessed both quantitatively and qualitatively. The adhesion and spreading of cells on materials were compared using Ti as a control. Present study indicates an improved cytocompatibility of DLC coated Ti in comparison to bare Ti.
Keywords: Diamond like carbon, cytotoxicity, fibroblast, osteoblast, endothelial cells, image analysis
Journal: Bio-Medical Materials and Engineering, vol. 12, no. 4, pp. 329-338, 2002
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