Searching for just a few words should be enough to get started. If you need to make more complex queries, use the tips below to guide you.
Article type: Research Article
Authors: Li, Guangjuna; ; | Shen, Wenb; | Chu, Minghuia | Mo, Guoweia | Yao, Liqina | Xu, Weidonga;
Affiliations: [a] Department of Orthopedics, Deqing People’s Hospital, Deqing, Zhejiang, China | [b] Department of Radiology, Deqing People’s Hospital, Deqing, Zhejiang, China
Correspondence: [*] Corresponding authors: Guangjun Li, Department of Orthopedics, Deqing People’s Hospital, Deqing, Zhejiang 313200, China. E-mail: joseph06113033@126.com. Weidong Xu, Department of Orthopedics, Deqing People’s Hospital, Deqing, Zhejiang 313200, China. E-mail: zjdqxwd@126.com
Note: [†] These authors contributed equally to this work.
Abstract: BACKGROUND:Calcium phosphate cements (CPCs) are biocompatible materials that have been evaluated as scaffolds in bone tissue engineering. At present, the stem cell density of inoculation on CPC scaffold varies. OBJECTIVE:The aim of this study is to analyze the effect of seeding densities on cell growth and osteogenic differentiation of bone marrow mesenchymal stem cells (BMMSCs) on a calcium phosphate cements (CPCs) scaffold. METHODS:BMMSCs derived from minipigs were seeded onto a CPC scaffold at three densities [1 million/mL (1M), 5 million/mL (5M) and 25 million/mL 25M)], and cultured for osteogenic induction for 1, 4 and 8 days. RESULTS:Well adhered and extended BMMSCs on the CPC scaffold showed significantly different proliferation rates within each seeding density group at different time points (P < 0.05). The number of live cells per unit area in 1M, 5M and 25M increased by 3.5, 3.9 and 2.5 folds respectively. The expression of ALP peaked at 4 days post inoculation with the fold-change being 2.6 and 2.8 times higher in 5M and 25M respectively as compared to 1M. The expression levels of OC, Coll-1 and Runx-2 peaked at 8 days post inoculation. CONCLUSIONS:An optimal seeding density may be more conducive for cell proliferation, differentiation, and extracellular matrix synthesis on scaffolds. We suggest the optimal seeding density should be 5 million/mL.
Keywords: Bone marrow mesenchymal stem cells, calcium phosphate cement, seeding density, osteogenic differentiation
DOI: 10.3233/BME-221394
Journal: Bio-Medical Materials and Engineering, vol. 34, no. 2, pp. 111-121, 2023
IOS Press, Inc.
6751 Tepper Drive
Clifton, VA 20124
USA
Tel: +1 703 830 6300
Fax: +1 703 830 2300
sales@iospress.com
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
IOS Press
Nieuwe Hemweg 6B
1013 BG Amsterdam
The Netherlands
Tel: +31 20 688 3355
Fax: +31 20 687 0091
info@iospress.nl
For editorial issues, permissions, book requests, submissions and proceedings, contact the Amsterdam office info@iospress.nl
Inspirees International (China Office)
Ciyunsi Beili 207(CapitaLand), Bld 1, 7-901
100025, Beijing
China
Free service line: 400 661 8717
Fax: +86 10 8446 7947
china@iospress.cn
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
如果您在出版方面需要帮助或有任何建, 件至: editorial@iospress.nl