Cytokines secretion from human mesenchymal stem cells induced by bovine bone matrix

Article type: Research Article

Authors: Rodríguez-Fuentes, Nayeli^a; | Alcántara-Quintana, Luz E.^b | Hernández-Ramírez, Diego F.^c | Piña-Barba, María C.^d | Cervantes-Uc, José M.^e | Núñez-Álvarez, Carlos A.^{c; f} | Ambrosio, Javier R.^f

Affiliations: [a] CONACYT, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, México | [b] CONACYT, Facultad de Enfermería y Nutrición, Universidad Autónoma de San Luis Potosí, San Luis Potosí, México | [c] Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Ciudad de México, México | [d] Instituto de Investigaciones en Materiales, UNAM, Ciudad de México, México | [e] Centro de Investigación Científica de Yucatán A.C., Mérida, Yucatán, México | [f] Facultad de Medicina, UNAM, Ciudad de México, México

Correspondence: [*] Corresponding author: Nayeli Rodríguez Fuentes, CONACYT, Centro de Investigación Científica de Yucatán, A.C. Calle 43 No. 130 x 32 y 34, Chuburná de Hidalgo, 97205, Mérida, Yucatán, México. Tel.: +52 999 942 83 30; Fax: +52 999 981 39 00; E-mails: nrodriguez@conacyt.mx, nayeli.rodriguez@cicy.mx, nayerodf@yahoo.com.mx

Abstract: BACKGROUND:Bovine bone matrix is a natural material that has been used in the treatment of bone lesions. In this study, bovine bone matrix Nukbone® (NKB) was investigated due its osteoconductive and osteoinductive properties. This biomaterial induces CBFA-1 activation and osteogenic differentiation, although the cytokines involved in these processes is still unknown. OBJECTIVE:The aim of this work was to determine the influence of NKB on the pro-osteoblastic and anti-osteoblastic cytokines secretion from human mesenchymal stem cells (hMSCs). METHODS:The hMSCs were cultured onto NKB and cytokines IL-2, IL-4, IL-6, IL-10, IL-12, IFN-γ and TNF-α were analized at 0-14 days by immunoassay. In addition, hemocompatibility of NKB and characterization of hMSCs were evaluated. RESULTS:NKB induces an increase on pro-osteoblastic cytokine secretion IL-4 and a decrease on anti-osteoblastic cytokine IL-6 secretion, at days 7 and 14 of cell culture. Interestingly, there was no statistical difference between secretion profiles of others cytokines analized. CONCLUSIONS:The up-regulation of IL-4 and down-regulation of IL-6, and the secretion profiles of other cytokines examined in this work, are findings that will contribute to the understanding of the role of NKB, and similar biomaterials, in bone homeostasis and in the osteoblastic differentiation of hMSCs.

Keywords: Bovine bone matrix, mesenchymal stem cell, bone regeneration, cytokine, nukbone

DOI: 10.3233/BME-218000

Journal: Bio-Medical Materials and Engineering, vol. 32, no. 4, pp. 217-228, 2021