Affiliations: Department of Orthopedic Surgery, Nara Medical University, Nara Japan | Biomaterials Research Group, University Hospital Leiden, Leiden, The Netherlands
Note: [] Requests for reprints should be addressed to Motoaki Okumura, Department of Orthopedic Surgery Nara Medical University, Kashihara City, Nara, 634, Japan.
Abstract: Porous alumina ceramics alone and combined with rat marrow cells were implanted subcutaneously in the back of syngeneic Fischer rats. Fluorochrome-labeling was performed post operatively and the ceramics were harvested 6 and 8 weeks after implantation. Undecalcified sections of the implants were observed under fluorescence microscopy and the de novo bone-ceramic interfacial areas were analyzed by a scanning electron microscope equipped with an electron probe microanalyzer. Alumina ceramics alone did not show any bone formation, while all marrow cell loaded ceramics showed new bone formation 6 and 8 weeks after implantation. Bone formation was first observed in the center of the pores and proceeded in a centrifugal direction, leading to contact with the ceramic. These results suggest that bone marrow cells have inherent osteogenic capacity and in the pore region of alumina ceramics progression of the osteogenesis causes the dissipation of intervening fibrous tissue between the de novo bone and alumina ceramic surface.
Keywords: alumina ceramics, bone marrow cell, osteogenesis, heterotopic bone formation, fluorochrome study
