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Article type: Research Article
Authors: Sönmezer, Dileka | Lati̇foğlu, Fatmab; | Toprak, Gülerb | Düzler, Ayhanb | İşoğlu, İsmail Alperc
Affiliations: [a] Çukurova University, Adana, Turkey | [b] Erciyes University, Kayseri, Turkey | [c] Abdullah Gül University, Kayseri, Turkey
Correspondence: [*] Corresponding author: Dr. Fatma Lati̇foğlu, Biomedical Engineering Department, Engineering Faculty, Erciyes University, 39039, Kayseri, Turkey. Tel.: +90 (352) 20 76 666, ext: 32 977; Fax: +90 (352) 43 75 784; E-mails: flatifoglu@erciyes.edu.tr, flatifoglu38@gmail.com
Abstract: BACKGROUND:The heart is surrounded by a membrane called pericardium or pericardial cavity. OBJECTIVE:In this study, we investigated the pericardial fluid (PF) for coating polycaprolactone (PCL) scaffolds. PFS, which is a PF component, was used for the coating material. In addition to using PFS for surface coating, MED and fetal bovine serum (FBS) were also used for comparison. METHODS:Pericardial fluid cells (PFSc) isolated from PF were cultured on coated PCL scaffolds for 1, 3, and 5 days. Cell viability was determined using 3-(4, 5-di-methylthiazol- 2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. RESULTS:The MTT assay results showed that the viability of cells on PCL scaffold coated with PFS increased over time (P < 0.005), and cell viability was significantly different between PCL scaffolds coated with PFS and non-coated PCL scaffolds. However, cell viability was significantly higher in the PCL scaffolds coated with PFS than non-coated and coated with FBS, MED, and PCL scaffolds. Scanning electron microscopy (SEM) microscopy images and MTT assay indicated that PFSc are attached, proliferated, and spread on PCL scaffolds, especially on PCL scaffolds coated with PFS. CONCLUSIONS:These results suggest that PFS is a biocompatible material for surface modification of PCL scaffolds, which can be used as a suitable material for tissue engineering applications.
Keywords: Pericardial fluid (PF), polycaprolactone (PCL), pericardial fluid structure, tissue engineering
DOI: 10.3233/BME-196014
Journal: Bio-Medical Materials and Engineering, vol. 32, no. 2, pp. 101-113, 2021
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