New tools for non-invasive exploration of collagen network in cartilaginous tissue-engineered substitute

Issue title: Selected papers from the 6th China–France International Symposium “Stem Cells and Regenerative Medicine” and the first meeting of the France–China International CNRS Network (GDRI) CeSMeR, Nancy, 10–13 July 2016

Guest editors: J.-F. Stoltz, J. Magdalou and D. Bensoussan

Article type: Research Article

Authors: Henrionnet, Christel^{a; *} | Dumas, Dominique^{a; b; *} | Hupont, Sébastien^b | Stoltz, Jean François^a | Mainard, Didier^{a; b; c} | Gillet, Pierre^a | Pinzano, Astrid^{a; **}

Affiliations: [a] Ingénierie Moléculaire et Physiopathologie Articulaire, UMR 7365 CNRS – Université de Lorraine, Bâtiment Biopôle, 9 avenue de la Forêt de Haye, BP 50184, F54505 Vandoeuvre-lès-Nancy, France | [b] Plateforme d’Imagerie Cellulaire PTIBC-IBISA, FR3209 CNRS, Faculté de Médecine, 9 avenue de la Forêt de Haye, BP 50184, F54505 Vandoeuvre-lès-Nancy, France | [c] Service de Chirurgie Orthopédique et Traumatologique, CHRU Nancy, 29 avenue du Maréchal de Lattre de Tassigny, CO60034, F54035 Nancy, France

Correspondence: [**] Corresponding author: Astrid Pinzano, Ingénierie Moléculaire et Physiopathologie Articulaire, UMR 7365 CNRS – Université de Lorraine, Bâtiment Biopôle, 9 avenue de la Forêt de Haye, CS50184, F54505 Vandoeuvre-lès-Nancy, France. Tel.: +33 372 746 565; Fax: +33 372 746 545; E-mail: astrid.pinzano@univ-lorraine.fr.

Note: [*] Equal contribution to this work.

Abstract: In tissue engineering approaches, the quality of substitutes is a key element to determine its ability to treat cartilage defects. However, in clinical practice, the evaluation of tissue-engineered cartilage substitute quality is not possible due to the invasiveness of the standard procedure, which is to date histology. The aim of this work was to validate a new innovative system performed from two-photon excitation laser adapted to an optical macroscope to evaluate at macroscopic scale the collagen network in cartilage tissue-engineered substitutes in confrontation with gold standard histologic techniques or immunohistochemistry to visualize type II collagen. This system permitted to differentiate the quality of collagen network between ITS and TGF-β1 treatments. Multiscale large field imaging combined to multimodality approaches (SHG-TCSPC) at macroscopical scale represent an innovative and non-invasive technique to monitor the quality of collagen network in cartilage tissue-engineered substitutes before in vivo implantation.

Keywords: Cartilage, tissue-engineered substitute, second harmonic generation, mesenchymal stem cells

DOI: 10.3233/BME-171645

Journal: Bio-Medical Materials and Engineering, vol. 28, no. s1, pp. S229-S235, 2017