Human umbilical cord derived matrix: A scaffold suitable for tissue engineering application

Issue title: Selected papers from the 6th China–France International Symposium “Stem Cells and Regenerative Medicine” and the first meeting of the France–China International CNRS Network (GDRI) CeSMeR, Nancy, 10–13 July 2016

Guest editors: J.-F. Stoltz, J. Magdalou and D. Bensoussan

Article type: Research Article

Authors: Dan, Pan^a | Velot, Émilie^a | Mesure, Benjamin^a | Groshenry, Guillaume^a | Bacharouche, Jalal^b | Decot, Véronique^{a; c} | Menu, Patrick^{a; *}

Affiliations: [a] UMR 7365, Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), CNRS–Université de Lorraine, Vandœuvre-lès-Nancy, 54505, France | [b] UMR 7564, Laboratoire de Chimie Physique et Microbiologie pour l’Environnement, Université de Lorraine, Villers-lès-Nancy, 54600, France | [c] Unité de Thérapie Cellulaire et Tissulaire, CHU de Nancy, Vandœuvre-lès-Nancy, 54500, France

Correspondence: [*] Corresponding author: Patrick Menu, UMR 7365 CNRS–Université de Lorraine, Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), 54505 Vandœuvre-lès-Nancy, France. E-mail: patrick.menu@univ-lorraine.fr; Tel.: +33 3 83 68 54 57; Fax: +33 3 83 68 54 09.

Abstract: Background:Human tissue derived natural extracellular matrix (ECM) has great potential in tissue engineering. Objective:We sought to isolate extracellular matrix derived from human umbilical cord and test its potential in tissue engineering. Methods:An enzymatic method was applied to isolate and solubilized complete human umbilical cord derived matrix (hUCM). The obtained solution was analyzed for growth factors, collagen and residual DNA contents, then used to coat 2D and 3D surfaces for cell culture application. Results:The hUCM was successfully isolated with trypsin digestion to acquire a solution containing various growth factors and collagen but no residual DNA. This hUCM solution can form a coating on 2D and 3D substrates suitable cell culture. Conclusion:We developed a new matrix derived from human source that can be further used in tissue engineering.

Keywords: Human matrix, tissue engineering, growth factors, residual DNA, stem cells

DOI: 10.3233/BME-171629

Journal: Bio-Medical Materials and Engineering, vol. 28, no. s1, pp. S95-S100, 2017