Stem cells cardiac patch from decellularized umbilical artery improved heart function after myocardium infarction

Issue title: Selected papers from the 6th China–France International Symposium “Stem Cells and Regenerative Medicine” and the first meeting of the France–China International CNRS Network (GDRI) CeSMeR, Nancy, 10–13 July 2016

Guest editors: J.-F. Stoltz, J. Magdalou and D. Bensoussan

Article type: Research Article

Authors: Li, Na^{a; b; c; *; **} | Huang, RanRan^{a; b; **} | Zhang, XiaoXia^{a; **} | Xin, Yi^{a; b} | Li, Jia^a | Huang, YiMin^{a; b} | Cui, Wei^{a; b} | Stoltz, Jean-Francois^{d; e} | Zhou, YuJie^{a; b} | Kong, QingYu^{a; b; *}

Affiliations: [a] Capital Medical University Affiliated Beijing Anzhen Hospital, Beijing, P.R. China | [b] Beijng Institute of Heart Lung and Blood Vessels Disease, Beijing, P.R. China | [c] The Key Laboratory of Remodeling-related Cardiovascular Diseases, Capital Medical University, Ministry of Education, Beijing, P.R. China | [d] CHU Nancy, Université de Lorraine et l’UMR CNRS IMOPA, Nancy, France | [e] CHU, Unité de Thérapie Cellulaire, 54511 Vandœuvre-Lès-Nancy, France

Correspondence: [*] Corresponding author: Na Li, QingYu Kong, Capital Medical University Affiliated Beijing Anzhen Hospital, Beijng Heart Lung and Vessels Disease Research Institute, 2 Anzhenlu, Chaoyang district, Beijing 100029, P.R. China. Tel.: +86 10 6445 6509; Fax: +81 10 6445 6095; E-mail: lina82002@126.com.

Note: [**] These authors contributed equally to this work.

Abstract: The construction of the high biocompatible biomaterials pretreated with MSC offers a promising strategy to improve the effects of stem cell therapy for the myocardial infarction (MI). However, assembling vascularized three-dimensional (3-D) myocardial tissues remains an enormous challenge. In this study, we optimized the decellularization protocol with the umbilical artery to construct microporous 3-D scaffold which is suitable for the stem cells (SC) proliferation. The SD rats underwent proximal left coronary ligation and a 5-mm diameter microporous SC patch was implanted directly on the infarct area (SC patch group). The LV contractile function, regional myocardial wall compliance, and tissue histology were assessed 4 weeks after patch implantation. The MSC patch integrated to the local heart tissue and the neo-vessels have been observed in the MSC patch. The vessels in the MSC patch were positive for the CD31 (marker for the mature endothelial cells). The left ventricle wall was thicker in the MSC patch group than the control group (p<0.05 vs. empty patch group). And the LVEF has been improved in the MSC patch group than empty patch group (59±6.7% vs. 31±4.5%, p<0.05). Conclusions:Our results showed that the implantation of the MSC patch improved cardiac contractile function in heart infarction rat model. The construction of artificial tissue from the decellularized umbilical artery and the MSC may open a promising perspective for the tissue therapy for MI.

Keywords: Mesenchymal stem cells, cardiac patch, umbilical artery decellularization, heart infarction

DOI: 10.3233/BME-171628

Journal: Bio-Medical Materials and Engineering, vol. 28, no. s1, pp. S87-S94, 2017