The enhancement of osteogenic capacity in a synthetic BMP-2 derived peptide coated mineralized collagen composite in the treatment of the mandibular defects
Affiliations: [a] Department of Traumatic Orthopaedics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong Province, China | [b] Department of Orthopaedics, Liaocheng People’s Hospital, Liaocheng Clinical School, Taishan Medical University, Liaocheng, Shandong Province, China | [c] Central Laboratory, Liaocheng People’s Hospital, Medical School of Liaocheng, Zhong Yuan Academy of Biological Medicine, Liaocheng University, Shandong Province, China | [d] Department of Clinical Chemistry and Haematology, University Medical Centre Utrecht, G03.550, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands
Note: [*] These authors contributed equally to this manuscript.
Abstract: The novel synthetic peptide P17-BMP-2 could promote cell attachment and enhance osteogenic capability. A composite, comprising nano-hydroxyapatite, collagen and poly(L-lactide) (nHAC/PLLA), was an efficient scaffold for carrier of P17-BMP-2. Our aim was to investigate whether nHAC/PLLA/P17-BMP-2 accelerates the osteogenesis as a reliable method for mandibular defect healing in this study. The repair capability was assessed by the gross observation, X-ray test and histological observation in four animal experiment groups at 2 week and 4 week after surgery: Group A (control), Group B (nHAC/PLLA treatment), Group C (nHAC/PLLA with 2 mg/g P17-BMP-2 treatment) and Group D (nHAC/PLLA with 10 mg/g P17-BMP-2 treatment). The Lane–Sandhu X-ray scores of the four groups were compared among four groups as well. The results showed that the composites containing the highest content of P17- BMP-2 performed best. Therefore, the nHAC/PLLA with P17-BMP-2 composite can accelerate the osteogenesis for mandibular defect healing and could be an ideal biological material as a bone graft material option for clinical applications.
