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Peroxynitrite induced fibrinogen site identification

Issue title: Frontiers in Biomedical Engineering and Biotechnology – Proceedings of the 4th International Conference on Biomedical Engineering and Biotechnology, 18–21 August 2015, Shanghai, China

Guest editors: Feng Liu, Dong-Hoon Lee, Ricardo Lagoa and Sandeep Kumar

Article type: Research Article

Authors: Luo, Yunjing; * | Shi, Jianlong | Li, Jingjing

Affiliations: College of Life Science and Bioengineering, Beijing University of Technology, 100124 P.R. China

Correspondence: [*] Address for correspondence: Yunjing Luo, College of Life Science and Bioengineering, Beijing University of Technology, 100 Ping Le Yuan, Chaoyang District, Beijing 100124, China. Tel.: +86-10-6739-1667; Fax: +86-10-67391667; E-mail: luoyj@bjut.edu.cn.

Keywords: Fibrinogen, LC-MS/MS, SDS-PAGE, peroxynitrite, tyrosine nitration

DOI: 10.3233/BME-151530

Journal: Bio-Medical Materials and Engineering, vol. 26, no. s1, pp. S2241-S2248, 2015

Published: 2015
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An increasing number of peroxynitrite-mediated fibrinogen nitrifications have been associated with thrombotic diseases. However, few reports related to priority nitrified fibrinogen injury sites exist. In this paper, an improved method, which simulated the environment in vivo, was used to inspect the structural changes of fibrinogen treated with peroxynitrite and LC-MS/MS in order to investigate the fibrinogen injury sites. The SDS-PAGE results indicated that γ subunits of Fg were vulnerable to oxidative/nitrative modifications induced by peroxynitrite. An in-depth analysis of fibrinogen γ chain (Fg I) nitration site identification and susceptibility to peroxynitrite-utilizing LC-MS/MS strategy was performed. Based on a large dataset, the results indicated the priority injury sites during database searching were Y96, Y262, Y274, Y348, and Y363; these results could be applied to biomedical studies.

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