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Multiplex biomarker analysis biosensor for detection of hepatitis B virus

Issue title: Frontiers in Biomedical Engineering and Biotechnology – Proceedings of the 4th International Conference on Biomedical Engineering and Biotechnology, 18–21 August 2015, Shanghai, China

Guest editors: Feng Liu, Dong-Hoon Lee, Ricardo Lagoa and Sandeep Kumar

Article type: Research Article

Authors: Xu, Huaa; b | Gu, Dayonga | He, Jian’ana | Shi, Leia | Yao, Jingyuc | Liu, Chunxiaoa | Zhao, Chunzhonga | Xu, Yunqinga | Jiang, Shengyangd; * | Long, June

Affiliations: [a] Institute of International Travel Health Care, Shenzhen Academy of Inspection and Quarantine, Shenzhen, 518033, P.R. China | [b] Shenzhen Children’s Hospital, Shenzhen, 518038, P.R. China | [c] Guangdong Medical college, Zhanjiang, 524023, P.R. China | [d] School of public health, Nantong University, Nantong, 226019, P.R. China | [e] Medical Inspection center, Zhujiang Hospital, Nan Fang Medical University, Guangzhou, 510280, P.R. China

Correspondence: [*] Address for corresponding: Shengyang Jiang, School of public health, Nantong University, Nantong, 226019, P.R. China. Tel.: 139-6291-5150; Fax: 0513-85228505; E-mail: syjiang@ntu.edu.cn. Jun Long, Medical Inspection center, Zhujiang Hospital, Nan Fang Medical University, Industrial Road No. 253, Guangzhou, Guangdong, P.R. China. Tel.: 020-61643491; Fax: 020-61643010; E-mail: 41671716@qq.com.

Keywords: Surface plasmon resonance, protein microarray, hepatitis B virus, label-free, biosensor

DOI: 10.3233/BME-151515

Journal: Bio-Medical Materials and Engineering, vol. 26, no. s1, pp. S2091-S2100, 2015

Published: 2015
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In this paper, we report the development of a protein microarray-based biosensor for the detection of the hepatitis B virus (HBV) serological markers using surface plasmon resonance (SPR Printing buffer, protein immobilization time and concentration of the capture protein were optimized systematically to determine the best performance of the biosensor. Under optimal conditions, five hepatitis B markers in 20 μL human serum can be simultaneously detected within 30 minutes, whereas other methods such as ELISA and PCR can detect only one marker within four hours. This platform has been validated by analysis of 35 patients known to have hepatitis B, with 85% agreement between the test platform and analysis by commercial enzyme-linked immunosorbent assay (ELISA) kits. The results demonstrate that the protein microarray with SPR displayed a sensitivity of 0.1 ng mL−1 for HBsAg. In addition to high sensitivity, it also shows excellent specificity, reproducibility and stability. This integrated protein microarray technique combined with SPR is a promising candidate for hepatitis B diagnosis with high-throughput.

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