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Glucose Biosensing Using Glassy Carbon Electrode Modified with Polyhydroxy-C60, Glucose Oxidase and Ionic-liquid

Issue title: Frontiers in Biomedical Engineering and Biotechnology – Proceedings of the 3rd International Conference on Biomedical Engineering and Biotechnology, 25–28 September 2014, Beijing, China

Article type: Research Article

Authors: Yang, Tian | Yang, Xiao-Lu | Zhang, Yu-Shuai | Xiao, BaoLin | Hong, Jun; ;

Affiliations: School of Life Sciences, Henan University, Kaifeng 475000, China | Institute of Biotechnology, Henan University, Kaifeng 475000, China

Note: [] Corresponding author: Jun Hong, School of Life Sciences, Henan University, Kaifeng 475000, China. Tel.: +86 13781161597; Fax: +86 371 23886258; E-mail: hongjun@henu.edu.cn.

Keywords: Glucose oxidase, polyhydroxy-C60, ionic liquid, direct electrochemistry

DOI: 10.3233/BME-141031

Journal: Bio-Medical Materials and Engineering, vol. 24, no. 6, pp. 2197-2202, 2014

Published: 2014
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Abstract

Direct electrochemistry of glucose oxidase (GOD) was achieved when an ionic liquid/GOD-Polyhydroxy-C60 functional membrane was confined on a glassy carbon electrode (GCE). The cyclic voltammograms (CVs) of the modified GCE showed a pair of redox peaks with a formal potential (E°') of − 329±2 mV. The heterogeneous electron transfer constant (ks) was 1.43 s-1. The modified GCE response to glucose was linear in the range from 0.02 to 2.0 mM. The detection limit was 1 μM. The apparent Michaelis-Menten constant (Kmapp) was 1.45 mM.

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